Composite

Part:BBa_K4743034:Design

Designed by: Jen Hsien, Liu   Group: iGEM23_PTSH-Taiwan   (2023-10-08)


Astaxanthin production set


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 8176
    Illegal PstI site found at 8266
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8176
    Illegal NheI site found at 627
    Illegal NheI site found at 915
    Illegal NheI site found at 1119
    Illegal NheI site found at 2274
    Illegal NheI site found at 9773
    Illegal NheI site found at 10061
    Illegal NheI site found at 10265
    Illegal PstI site found at 8266
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8176
    Illegal BglII site found at 725
    Illegal BglII site found at 5611
    Illegal BglII site found at 9871
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 8176
    Illegal PstI site found at 8266
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 8176
    Illegal PstI site found at 8266
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 4377
    Illegal BsaI.rc site found at 12941


Design Notes

No


Source

No

References

1.Jin, J., Wang, Y., Yao, M., Gu, X., Li, B., Liu, H., Ding, M., Xiao, W., & Yuan, Y. (2018). Astaxanthin overproduction in yeast by strain engineering and new gene target uncovering. Biotechnology for biofuels, 11, 230. https://doi.org/10.1186/s13068-018-1227-4

2.Chang, J. J., Ho, C. Y., Ho, F. J., Tsai, T. Y., Ke, H. M., Wang, C. H., Chen, H. L., Shih, M. C., Huang, C. C., & Li, W. H. (2012). PGASO: A synthetic biology tool for engineering a cellulolytic yeast. Biotechnology for biofuels, 5(1), 53. https://doi.org/10.1186/1754-6834-5-53